digital ventilated cages dvc  (Tecniplast inc)

Journal: Life

Article Title: Sex-Specific Adaptations in Alzheimer’s Disease and Ischemic Stroke: A Longitudinal Study in Male and Female APP swe /PS1 dE9 Mice

doi: 10.3390/life15030333

Figure Lengend Snippet: Overview of the study design. This longitudinal study investigated the impact of stroke and Alzheimer’s disease on cognitive impairment, emphasizing sex differences. Male and female WT and APP swe /PS1 dE9 (APP/PS1) mice (3-month-old) were included. Baseline physiological parameters (body weight (BW) and systolic blood pressure (SBP)) were monitored. At 3.5 months of age, mice underwent either right transient middle cerebral artery occlusion (tMCAO) or sham surgery, resulting in eight groups: (1) male WT sham, (2) male WT stroke, (3) male APP/PS1 sham, (4) male APP/PS1 stroke, (5) female WT sham, (6) female WT stroke, (7) female APP/PS1 sham, and (8) female APP/PS1 stroke. Body weight and systolic blood pressure were monitored monthly post-stroke. Walking patterns of each mouse were individually monitored 24/7 for eight months using digital ventilated cages (DVCs). Magnetic resonance imaging (MRI) was conducted at 0.5, 4, and 8 months post-stroke. At 12 months of age (8 months post-stroke), spatial learning and memory were assessed using the Morris water maze (MWM) test. After the final neuroimaging session, mice were sacrificed, and brains were collected for post-mortem analysis, including immunohistochemical stainings, polarized light imaging, and biochemistry.

Article Snippet: In this study, we utilized digital ventilated cages (DVCs) (Tecniplast S.p.A., Buguggiate (VA), Italy), which are equipped with sensing boards to continuously monitor the activity and location (centroids) of individually housed mice [ ].

Techniques: Magnetic Resonance Imaging, Immunohistochemical staining, Imaging